To evaluate the anti-tumor efficacy of CAR-T cells, we quantified the tumor cells every 4 days and observed no detectable Raji cells in the culture of 28z/IL-7 CAR-T cells for 30 days, while the conventional CAR-T cells controlled Raji cell growth for the first 10 days, but lost anti-tumor efficacy after 14 days re-challenge (Fig. 4B). The gene discussed is IL7; the disease is neoplasm.