Indeed, we observed that the CD8 + 28z/IL-7-CAR-T cell numbers were maintained at a stable level, but 28z-CAR-T cells were detected as 80% CD8 + T cells at the end of their lifetime in culture but no efficacy to eliminate tumor cells (Fig. 4B, Supplementary Fig. 4). The gene discussed is CD8A; the disease is neoplasm.