In addition, while the most frequent complication of thymoma is anti-acetylcholine receptor antibody-positive myasthenia gravis, the increase of the CHRNA1 expression was milder than other neuromuscular antigens such as GABRA5 and RYR3. The moderate increase of CHRNA1 expression may be sufficient for causing serious symptoms due to the accessibility of acetylcholine receptor antibodies54 and the importance of their biological functions55. This evidence concerns the gene RYR3 and thymoma.