To corroborate the above data and determine the level of importance SMOC2 has on the events of EMT in RCC cells, we transfected ACHN and 786-O cells with either a control scrambled siRNA (ssiRNA) or SMOC2 siRNA to reduce the expression of SMOC2 (Fig. 9a, b), and monitored any potential changes in the induction of EMT by TGFβ1. The gene discussed is SMOC2; the disease is renal cell carcinoma.