Consistently, these results were also confirmed by IHC in HCC samples, which demonstrated that the expression of RBCK1 was significantly higher in HCC than in the adjacent normal tissues (Fig. 7A, B) and that higher IHC scores of RBCK1 were positively associated with shorter tumor-free survival and overall survival (Fig. 7C). Here, RBCK1 is linked to hepatocellular carcinoma.