To shed light on the mechanisms underlying the progression of luminal cells, we performed droplet-based scRNA-seq on dissociated Pten(i)pe−/− prostates at 6 and 15 months AGI, corresponding to intermediate-stage PINs and malignant tumors, respectively, and combined these datasets with those obtained from early- (3 months AGI; see above) and late-stage [9 months AGI (17)] PIN lesions. This evidence concerns the gene PTEN and cancer.