To elucidate the transcriptomic changes that occur in various cell types of early PIN lesions, we performed droplet-based single-cell RNA-sequencing (scRNA-seq) on 9956 and 7122 cells isolated from prostates of PtenL2/L2 (control) and Pten(i)pe−/− mice, respectively, 3 months AGI. Here, PTEN is linked to prostate intraepithelial neoplasia.