For this purpose, BmE cells and NPC1-null cells were inoculated with virus VP39 labeled with enhanced green fluorescent protein (VP39-EGFP) at a multiplicity of infection (MOI) of 80 at 4°C for 1h, then shifted to 28°C for 2 h post-virus inoculation before being imaged by confocal microscopy. This evidence concerns the gene NPC1 and infection.