Prepared Fe3O4/Cs were evaluated as anticancer agent for HCC DEN-induced rats, where PI3K/Akt/mTOR and MAPK (ERK, JNK, P38) signaling pathways were studied, as well as inflammatory markers (TLR4, iNOS and TNF-α), cleaved caspase-3, and liver enzyme (ALT, AST and GGT) activities. The gene discussed is TLR4; the disease is hepatocellular carcinoma.