In our study, no significant correlation was found between pathological type, histological grade, percentage of tumor stroma infiltrating lymphocyte, lymph-vascular invasion, estrogen receptor, progesterone receptor, human epidermal growth factor 2, Ki67, molecular subtypes, and NSLN metastasis, which is different from relevant literature reports.[26–30] The purpose of this study is to provide an important reference value for surgeons considering whether SLN positive breast cancer patients can omit ALND or not. The gene discussed is ESR1; the disease is breast cancer.