NLRP3-mediated cardiomyocyte pyroptosis is critical for the initiation and development of DCM, so we measured the protein expression levels of NLRP3, p-caspase-1 (pro-caspase-1), c-caspase-1 (cleaved-caspase-1), GSDMD, GSDMD-N, IL-18, and IL-1β in mice heart tissue by Western blot. Here, GSDMD is linked to familial dilated cardiomyopathy.