Here, SLC6A14 depletion provokes amino acid starvation followed by the cripple of the PI3K/AKT/mTORC1 pathway, thus raising the possibility that SLC6A14 can be treated as an amino acid sensor to promote GC metastasis through sensitizing the PI3K/AKT/mTORC1 pathway. Here, SLC6A14 is linked to gastric cancer.