Subsequently, our analysis of the dataset of pancreatic cancer single-cell sequencing revealed that the glycolytic pathway was mainly activated in epithelial cells, and seven genes in the gene signature were also mainly expressed in epithelial cells, especially ITGB6, LY6D, and MET, suggesting that epithelial cells may play an important role in the progression of PC. This evidence concerns the gene ITGB6 and pachyonychia congenita.