To disclose the impacts of CCL20 and TGF-β on ICB therapy in different immune tumor microenvironments, we divided the urothelial cancer cohort into 3 groups according to the IHC score of PD-L1 expression on immune cells and compared the expression level of CCL20 and TGF-β in CR/PR and SD/PD subgroups. The gene discussed is CD274; the disease is neoplasm.