To further define the functional significance of VIPR1 in HCC, we examined VIPR1 mRNA levels in the human hepatocyte cell line (LO2) and seven human HCC cell lines by performing RT-qPCR analysis (Fig. S2A), and identified three relatively VIPR1 high-expression cell lines, namely Hep3B, HepG2, and Huh-7. This evidence concerns the gene VIPR1 and hepatocellular carcinoma.