OTUB1 and pachyonychia congenita: miR-23a-3p was overexpressed or knocked down in PC cells by transfecting the miR-23a mimics or inhibitors into the cells (Supplementary Figure 4A), and mRNA and protein expression of OTUB1 was detected by qRT–PCR and western blot assay (Figures 8(e) and 8(f)).