First, HT115 and RKO CRC cell lines with low levels of MAGI3 (Additional file 4: Fig. S2A) were stably transfected with MAGI3 (Additional file 4: Fig. S2B), and the data showed that overexpression of MAGI3 significantly inhibited CRC cell proliferation (Fig. 2a) and colony formation (Fig. 2b). This evidence concerns the gene MAGI3 and colorectal carcinoma.