FAM53B and familial pancreatic carcinoma: We cultured a normal pancreatic ductal epithelial cell line (HPDE) and four pancreatic cancer cell lines (SW 1990, BxPC-3, CFPAC-1, and PANC-1), extracted RNA from the cells, and performed qRT-PCR to determine the expression profiles of key prognostic genes (ANLN, ARNTL2, CDKN3, and FAM53B) and RNA methylation regulators.