Indeed, both 29F.1A12 and RMP1-30 reactivity to live WT B16-F10 cells was > 3-fold increased in 3D versus 2D cultures (Fig. 5c,d), consistent with variations in tumor cell-intrinsic PD-1 levels under distinct culture conditions reported by others15,17,29. The gene discussed is PDCD1; the disease is neoplasm.