The absence of the C-terminal transmembrane region (Δ1170–1250) and low-complexity region (Δ540–640), but not the C2 region (Δ720–820), abolished the inhibitory effects of OTOF on HIV-1 infection (Fig. S6B and C); this indicates that the low-complexity domain is crucial for the antiviral activity of OTOF and the antiviral activity of OTOF relies on its translocation into the cell membrane. The gene discussed is OTOF; the disease is HIV-1 infection.