Consistent with the RNAseq data from young Prdm16flox/flox; Mesp1-Cre mice, we confirmed that many genes that are implicated in cardiac hypertrophy and oxidative stress were upregulated or downregulated in the cardiac tissues of 1-month-old, male Prdm16CKO compared with control (S3 Fig in S1 File). Here, MESP1 is linked to cardiac hypertrophy.