Here, we recruited a patient with familial HCM who showed progressive left ventricular systolic dysfunction leading to advanced heart failure, carrying a heterozygous TNNT2 Δ160E mutation; we then generated a set of isogenic induced pluripotent stem cell (iPSC) clones with 3 distinct genotypes using CRISPR/Cas9 genome editing, and examined their potential as a disease model for HCM. This evidence concerns the gene TNNT2 and heart failure.