The antibodies used in IHC for NCL detection in the cancer tissues were from different clones with a variety of binding epitopes binding sites, binding to amino acids 2–17 [16] and 271–520 [18] of the 710 amino acids NCL protein or undefined NCL epitope using whole human NCL protein from Raji cell extract as the immunogen for the antibody production [13–15, 21–23, 46] or antibody clones that did not provide the antigen binding site [19, 20, 47]. This evidence concerns the gene NUCLEOLIN and cancer.