Moreover, overexpression of total NCL protein detected by IHC was reported to be significantly associated with poor OS [15, 16, 18] and DFS [13–15, 47] in pediatric ependymoma, hepatocellular carcinoma, pancreatic ductal adenocarcinoma, and non-small cell lung cancer, but no significance with patient outcome was reported in the studies with regards to ependymoma, hepatocellular carcinoma, and pancreatic ductal adenocarcinoma [14, 19, 20]. This evidence concerns the gene NUCLEOLIN and hepatocellular carcinoma.