In the present study, we found miR-26a-5p exerted antitumor effects on NSCLC by inhibiting cancer stem-cell like properties through downregulating the DNMT3A to increase SFRP1 expression and then increasing SFRP1 inhibited Wnt/β-catenin pathway in the regulatory process (Figure 7). The gene discussed is SFRP1; the disease is cancer.