In order to reveal in detail that sinobine hydrochloride reversed tumor-derived DNA-induced apoptosis reduction of HCC cells, we examined the protein Caspase 3 levels, and the results were consistent with flow cytometry results, suggesting that siCXCR4 or siCCR7 reversed tumor-derived DNA-induced apoptosis reduction of HCC cells. This evidence concerns the gene CASP3 and hepatocellular carcinoma.