Lastly, we included CD73 in the analysis since this molecule plays an important role in Treg cells’ function and in tumor progression (24, 25), observing that the abundance of CD73+ cTreg and Tr1 cells are equivalent in naive and TdLN, ∼90% and ∼80%, respectively (Figure 3C, bottom panel), but in TILs, the frequencies of Tr1 cells (∼90%) are higher than cTreg cells (∼70%). The gene discussed is NT5E; the disease is neoplasm.