Tumor draining lymph nodes (TdLN) and tumor-infiltrating lymphocytes (TILs) were harvested from tumor-bearing mice (or inguinal lymph nodes from control naive animals) at day 20 post-tumor injection, and the expression of CD45, CD4, Foxp3, CD49b, and IL-10 was analyzed by flow cytometry. The gene discussed is PTPRC; the disease is neoplasm.