To define whether YAP activation participates in EMT, migration and invasion of ESCC cells, we performed cell transfection to introduce constitutively active YAP (CA-YAP; pCMV-Flag S127A YAP), compelling YAP to be retained in nuclei whereby cells achieve an increased total output of YAP activity, into KYSE30 cells with or without C12orf59 overexpression. The gene discussed is TMEM52B; the disease is esophageal squamous cell carcinoma.