Furthermore, the expression of C12orf59 in human ESCC cell lines was also determined by evaluating the mRNA and protein levels of C12orf59 in human immortalized esophageal cells (NE1) and eight ESCC cell lines (KYSE30, KYSE140, KYSE150, KYSE180, KYSE410, KYSE450, KYSE510, and TE-1). Here, TMEM52B is linked to esophageal squamous cell carcinoma.