It has also been shown that knockdown of Gasdermin-D (GSDMD) or inhibition of GSDMD with GSDMD-C, afenide, etc. can effectively reduce the secretion of mature IL-1β and IL-18 and microglia pyroptosis and subsequent ischemic stroke-related brain injury (Zhang et al., 2019b; Han C. et al., 2020; Wang K. et al., 2020). This evidence concerns the gene GSDMD and ischemic stroke.