SAE1 and hepatocellular carcinoma: However, except cg07931151, cg16857335, cg24045565, and cg26870061 (where no difference was observed in the methylation level between tumor and normal samples), significant hypermethylation was observed in the remaining seven CpG sites of SAE1 in tumors compared with normal tissues (Figure 2b), suggesting that DNA methylation was not likely to be the factor that caused the elevated expression of SAE1 in HCC.