Subsequently, 4 genes (Htr2c, Gsk3b, Nrxn1, and Nr3c1) were selected for qPCR validation according to the following criteria: (1) upregulated genes on RNA-seq; (2) predicted target genes of miR-137-3p; and (3) relevant to neuronal function or psychiatric disorders. Here, NRXN1 is linked to psychiatric disorder.