In DhMGs involved in AML signaling, 5hmC was significantly enriched in transcription factors (CEBPA, NFKB1, RARA, and SPI1), tyrosine kinases (FLT3 and KIT), the growth factor KITLG, phosphoinositide 3-kinase (PI3K) family members (PIK3C2B, PIK3CD, and PIK3R1) and the cell cycle regulator CCNA1 in AML patients. The gene discussed is SPI1; the disease is acute myeloid leukemia.