Besides, western blot assay of the expression of RAC1 and MG53 in the matched HCC specimens further verified that the expression levels of MG53 was significantly negatively correlated with those of RAC1 (Fig. 2G, H), which verified the negative regulation of RAC1 by MG53 in clinical HCC specimens. The gene discussed is TRIM72; the disease is hepatocellular carcinoma.