To further investigate the role of methylation in SLC5A7 inactivation in CRC cells, siRNA targeting DNMT1, DNMT3a, and DNMT3b was used and shown to efficiently inhibit DNMT1, DNMT3a, and DNMT3b expression (Additional file 1: Fig. S1). This evidence concerns the gene SLC5A7 and colorectal carcinoma.