We assessed autophagy activity in 35-day-old iPSC-derived neurons (herein abbreviated as idNeurons) expressing specific markers such as βIII-tubulin, Map2, and NeuN (Figure S1D), and again we observed a significant reduction of p62 and a parallel increase of LC3-II in both LHON-affected carrying the 3460 and the 11778 mutations (Figure 1G). This evidence concerns the gene SQSTM1 and Leber hereditary optic neuropathy.