Moreover, the CRC sublines that were subjected to prolonged exposure to 5‐FU or Oxa for the establishment of a preclinical model of PD‐L1high CRC displayed upregulation of PD‐L1 expression and CSC‐like properties, which were significantly abrogated by a PD‐L1 neutralising Ab. Here, CD274 is linked to colorectal carcinoma.