As shown by immunofluorescence (Figure 1I, left) and real‐time PCR (Figure 1I, right) analyses, the CD133highCD44high subpopulation within the three CRC cell lines exhibited significantly greater expression of PD‐L1 protein and CD274 (which encodes PD‐L1) mRNA levels compared with their corresponding CD133lowCD44low population. Here, CD274 is linked to colorectal carcinoma.