We found that silencing CD274 expression resulted in decreases in the expression of CSC markers, sphere‐forming ability and colony formation in the presence of 5‐FU and sensitised cells to 5‐FU‐induced apoptosis, implying the involvement of PD‐L1 in chemoresistance and CSC properties in CRC cells (Figure S2E–H). This evidence concerns the gene CD274 and colorectal carcinoma.