Because IFN‐γ stimulation induces functional PD‐L1 in tumour cells,47 we further analysed FACS‐sorted PD‐L1low and PD‐L1high populations after stimulation with IFN‐γ and found consistent results that PD‐L1high subpopulations in HCT116, HT‐29 and CT26 cells exhibited significantly reduced sensitivity to 5‐FU/Oxa treatment and greater capacities for sphere formation compared with PD‐L1high subpopulations in their corresponding parental cells (Figure S1G,H). This evidence concerns the gene CD274 and neoplasm.