To further clarify the functional importance of PTEN in NSCLC cells, it was next knocked down with an appropriate shRNA construct (shPTEN) or overexpressed with an appropriate plasmid (OE‐PTEN) in HCC827 and H1299 cells, with Western blotting and qPCR being used to confirm successful knockdown or overexpression of this gene at the mRNA and protein levels (Figure 6A,B). This evidence concerns the gene PTEN and non-small cell lung carcinoma.