Using RNA-sequencing data from the parental isolates, we found that BgTH12-04537/BgTH12-04538 and Bgt-51729/Bgt-51731 are highly expressed at early stages of infection, corresponding to the establishment of the haustorial feeding structure at 2 d post infection (dpi), reminiscent of other wheat mildew Avr genes (SI Appendix, Figs. S4 and S5). Here, NLRP6 is linked to infection.