Transfection with CpGf-AFP-sr39 resulted in 43 ± 3– and 50 ± 9–fold higher accumulation in Huh7 and Hep3b over THLE-3, and this HCC specificity was not observed with CpGf-CMV-sr39 (Fig. 5, F and G). The gene discussed is AFP; the disease is hepatocellular carcinoma.