In asparagine-free medium, tumor cell enrichment in [U-13C5]-l-glutamine and in glutamine-derived glutamate (M + 5) and aspartate (M + 4) peaked at 6 hours after addition of the tracer, consistent with a metabolic channeling of intermediates of oxidative metabolism of glutamine from glutaminase (GLS) to GOT2 (Fig. 1I). This evidence concerns the gene GLS and neoplasm.