In contrast, both MKN-45 and BGC-823 were resistant to arginine deiminase (ADI) treatment due to the expression of ASS gene, even when the drug concentration reached 1 U/mL, which was about 400-fold higher than the dosage used on two sensitive cervical cancer cell lines, C33-A and SiHa [16] (Table 1). Here, ASS1 is linked to cervical carcinoma.