Additionally, cognitive impairment and anxiety-like behavior induced by cerebral ischemia could be alleviated by puerarin due to the activation of the PI3K/Akt1/GSK-3β/ myeloid cell leukemia-1 (MCL-1) signaling pathway, and the reduction of MDA, GSH-Px and thiol levels in the hippocampus and frontal cortex [83]. This evidence concerns the gene AKT1 and brain ischemia.