As shown inFigure 4, loss of PSD-95-ir puncta/synapses was apparent in all measured regions in 3xTg-AD sedentary mice versus Ntg sedentary controls (three-way ANOVA, genotype × running × region, F[1,60] = 121.2, P< 0.0001; post hoc test, ** P< 0.01). This evidence concerns the gene DLG4 and Alzheimer disease.