Finally, we were able to demonstrate a strong intrinsic variation of AKT phosphorylation by immunohistochemical staining of phosphorylated AKT in tissue samples from patients with squamous-cell lung-cancer characterized by either FGFR1 amplification (n = 33) or FGFR1 protein overexpression (n = 50) (Fig. 2h–j, Table 1 and Supplementary Table 3). Here, AKT1 is linked to squamous cell lung carcinoma.