Finally, we found that TGF-β expression level was inversely correlated with HIC1 in the cell lines commonly used in prostate cancer research (Fig. 2g, R2 = 0.421), especially in the three malignant cell lines, including C4-2B, PC3, and DU145 (Fig. 2h). This evidence concerns the gene TGFB1 and Familial prostate cancer.