NKX3-1 and posterior cortical atrophy: After 40 weeks of age, the dCKO mice exhibited obvious metastases where PCa cells spread to distant locations such as the lung, para-aortic lymph nodes (LNs), and adrenal glands, as verified by staining for androgen receptor (AR), NKX3.1, and/or Ki-67 (Fig. 1f, g, Supplemental Fig. S1f–i).