Prevalent genetic alterations in CRC such as inactivating mutations in FBXW7 (p.Try545Cys and p.Arg479*), APC (p.Thr621fs and p.Ser1415fs), and TP53 (p.Arg306*) as well as activating mutations in KRAS (p.Gly12Phe) and PIK3CA (p.Glu545Gly) were detected in all tumors with variant allele frequencies (VAFs) of ~0.5 except for TP53 [variant allele frequency (VAF) = ~1.0]. The gene discussed is PIK3CA; the disease is colorectal carcinoma.