ZCCHC4 and hepatocellular carcinoma: Consistently, the immunohistochemistry (IHC) analysis showed that the protein level of ZCCHC4 was significantly higher in HCC tissues, with 73% (47/64) of HCC tissues showing increased ZCCHC4 expression compared to corresponding non-tumor tissues (Fig. 1c, d and Supplementary Fig. S1b, c).