Next, we measured the effects of knocking down DLEU1 on multiple malignant behaviors of cancer cells and found that when compared to control shRNA (shNC)-transfected cells, shDLEU1#2 or #3 cells significantly reduced short-term cell proliferation (Fig. 2B), long-term proliferation (Fig. 2C), migration (Fig. 2D), and invasion (Fig. 2E), suggesting that DLEU1 critically controls multiple malignant phenotypes of breast cancer cells. This evidence concerns the gene DLEU1 and cancer.