Knockdown of APPL1/2 with siRNA resulted in reduced cell numbers when compared with PC-3U control cells treated with EGF only (Figure S5c), suggesting that APPL proteins are important for proliferation or survival of EGF-stimulated cells, consistent with the observation of increased APPL1 gene expression and protein expression during the initiation and progression of prostate cancer.16 This evidence concerns the gene APPL1 and prostate cancer.