In the present study, we report that deletion of CaV2.1 from serotonergic neurons in the DRN results in increased aggressive behavior in the RI and tube displacement test (Fig. 3), enhanced firing rates of putative serotonergic neurons (Fig. 4) and increasing c-fos expression in the DRN and VHMvl (Fig. 5) in male ePet-Cre/Cacna1a−/− compared with Cacna1a+/+ mice, while anxiety-like behavior is not affected. This evidence concerns the gene FOS and Anxiety.