Our study confirmed the roles of SssP1 in these three hallmark features in vivo by detecting the red blood cell count, white blood cell count, mRNA level of IL-8 encoding gene, bacterial load in CFS and brain, and pathological injury of the brain during meningitis caused by S. suis (Fig 8). This evidence concerns the gene CXCL8 and myalgic encephalomeyelitis/chronic fatigue syndrome.