To further corroborate that PRC2 inactivation in various cancer contexts was associated with a cold TME, we identified available archival tumor tissues with confirmed PRC2 inactivation through EED or SUZ12 loss-of-function mutations by MSK-IMPACT and loss of H3K27me3 immunostaining by IHC (Supplemental Table 3 and Supplemental Figure 1F). Here, EED is linked to neoplasm.