P2X4 expressed in different cell types MNs, microglia or macrophage involved in ALS pathogenesis and neurodegeneration [9, 45, 46, 63, 64] may promote either beneficial or detrimental effects in specific cell types and/or at different stages of ALS progression since changes in the expression of microglial P2X4 (our results Fig. 4, S8 and [46]) and in ATP concentration were observed during ALS progression[45]. This evidence concerns the gene P2RX4 and amyotrophic lateral sclerosis.